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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
25/07/2012 |
Data da última atualização: |
25/07/2012 |
Autoria: |
SILVA, C. S. da; PEREIRA, D. D.; SARMENTO, M. B.; SAMPAIO, T. G. |
Título: |
Isolamento de rizobactérias e efeito da bacterização de sementes na promoção do crescimento de tomateiro. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Revista Científica Rural, Bagé, v. 13, n. 1, p. 170-183, ago. 2011. |
ISSN: |
1413-8263 |
Idioma: |
Português |
Palavras-Chave: |
Análise fisiológica; Crecimento; Cultivar Kombat; Cultivar Rio Grande; Rizobactéria; Solanum lycopersicon. |
Thesagro: |
Germinação; Semente; Tomate. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 00784naa a2200265 a 4500 001 1929232 005 2012-07-25 008 2011 bl uuuu u00u1 u #d 022 $a1413-8263 100 1 $aSILVA, C. S. da 245 $aIsolamento de rizobactérias e efeito da bacterização de sementes na promoção do crescimento de tomateiro. 260 $c2011 650 $aGerminação 650 $aSemente 650 $aTomate 653 $aAnálise fisiológica 653 $aCrecimento 653 $aCultivar Kombat 653 $aCultivar Rio Grande 653 $aRizobactéria 653 $aSolanum lycopersicon 700 1 $aPEREIRA, D. D. 700 1 $aSARMENTO, M. B. 700 1 $aSAMPAIO, T. G. 773 $tRevista Científica Rural, Bagé$gv. 13, n. 1, p. 170-183, ago. 2011.
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Embrapa Hortaliças (CNPH) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
20/01/2022 |
Data da última atualização: |
20/01/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; DAVID VILLAR-ÁLVAREZ, CSIC; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, CSIC; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, CSIC. |
Título: |
Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Viruses , v.13, n.12, 2021. |
ISSN: |
1999-4915 |
DOI: |
https://doi.org/10.3390/v13122498 |
Idioma: |
Inglês |
Conteúdo: |
Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. |
Thesagro: |
Vírus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02107naa a2200217 a 4500 001 2139210 005 2022-01-20 008 2021 bl uuuu u00u1 u #d 022 $a1999-4915 024 7 $ahttps://doi.org/10.3390/v13122498$2DOI 100 1 $aLEASTRO, M. O. 245 $aSpontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage.$h[electronic resource] 260 $c2021 520 $aPrevious results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. 650 $aVírus 700 1 $aVILLAR-ÁLVAREZ, D. 700 1 $aASTUA, J. de F. 700 1 $aKITAJIMA, E. W. 700 1 $aPALLÁS, V. 700 1 $aSÁNCHEZ-NAVARRO, J. Á. 773 $tViruses$gv.13, n.12, 2021.
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